Entitled “Engineering 3D micro-compartments for highly efficient and scale-independent expansion of human pluripotent stem cells in bioreactors” this paper highlights:
– Biomimetic features of C-Stem™-generated capsules, allowing for in vivo-like hiPSC self-organization into lumenized rosettes
– Unprecedented exponential hiPSC growth in 10L bioreactors: >275-fold within 6.5 days, with high reproducibility
– Maintenance of cell quality (pluripotency & genonomic integrity) throughout massive expansion