{"id":941,"date":"2023-01-07T12:49:00","date_gmt":"2023-01-07T12:49:00","guid":{"rendered":"https:\/\/preprod.treefrog.fr\/stemcelljungle\/?p=941"},"modified":"2023-02-14T11:07:44","modified_gmt":"2023-02-14T11:07:44","slug":"how-do-human-pluripotent-stem-cells-grow-in-vivo","status":"publish","type":"post","link":"https:\/\/preprod.treefrog.fr\/stemcelljungle\/how-do-human-pluripotent-stem-cells-grow-in-vivo\/","title":{"rendered":"How do human pluripotent stem cells grow in vivo ?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"941\" class=\"elementor elementor-941\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-778bd0da elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"778bd0da\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-428bc3e2\" data-id=\"428bc3e2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7f83149 elementor-widget elementor-widget-heading\" data-id=\"7f83149\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\"><font color=\"#000000\">Human primed pluripotency: a 7-day window<\/font><br><\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3bc6364 elementor-widget elementor-widget-text-editor\" data-id=\"3bc6364\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Pluripotency is a transient cell property <i>in vivo<\/i>. At the end of the first week of development, na\u00efve hPSCs transition to primed pluripotency. Within the following 5 to 7 days, the hPSC colony, benefiting from a very fast 15h to 16h cell cycle, grows exponentially to reach over 1,000 cells. Following this exponential amplification, hPSCs exit pluripotency as they commit to one of the three germ layers that undergo gastrulation. Overall, among the 40 cell divisions required to constitute an adult body (~37 trillion cells), 10 are estimated to occur within the second week of development, highlighting the importance of the pluripotent stage.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4b8c3a1 elementor-widget elementor-widget-image\" data-id=\"4b8c3a1\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1920\" height=\"1080\" src=\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/Human-primed-pluripotency-in-vivo-TreeFrog-In-The-Stem-Cell-Jungle-web.jpg\" class=\"attachment-full size-full wp-image-1833\" alt=\"Human primed pluripotency in vivo TreeFrog In The Stem Cell Jungle\" srcset=\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/Human-primed-pluripotency-in-vivo-TreeFrog-In-The-Stem-Cell-Jungle-web.jpg 1920w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/Human-primed-pluripotency-in-vivo-TreeFrog-In-The-Stem-Cell-Jungle-web-300x169.jpg 300w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/Human-primed-pluripotency-in-vivo-TreeFrog-In-The-Stem-Cell-Jungle-web-1024x576.jpg 1024w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/Human-primed-pluripotency-in-vivo-TreeFrog-In-The-Stem-Cell-Jungle-web-768x432.jpg 768w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/Human-primed-pluripotency-in-vivo-TreeFrog-In-The-Stem-Cell-Jungle-web-1536x864.jpg 1536w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/Human-primed-pluripotency-in-vivo-TreeFrog-In-The-Stem-Cell-Jungle-web-700x394.jpg 700w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/Human-primed-pluripotency-in-vivo-TreeFrog-In-The-Stem-Cell-Jungle-web-539x303.jpg 539w\" sizes=\"(max-width: 1920px) 100vw, 1920px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dfc15c5 elementor-widget elementor-widget-text-editor\" data-id=\"dfc15c5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><strong>Human primed pluripotency in vivo: a \u00a07 day-window, a protected microenvironment, and a 3D epithelial organization.<\/strong><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9412ca5 elementor-widget 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!important;}[data-css=\"tve-u-18636929c15\"]{--tve-alignment:center;float:none;max-width:516px;margin:0px auto !important;padding:20px !important;}[data-css=\"tve-u-18636929c16\"]{border-radius:50px;overflow:hidden;box-shadow:none;--tve-border-radius:50px;background-color:rgb(21,237,163) !important;--background-color:rgb(21,237,163) !important;--tve-applied-background-color:rgb(21,237,163) !important;}:not(#tve) [data-css=\"tve-u-18636929c15\"]:hover [data-css=\"tve-u-18636929c16\"]{background-color:rgb(0,171,105) !important;--background-color:rgb(0,171,105) !important;--tve-applied-background-color:rgb(0,171,105) !important;}[data-css=\"tve-u-18636929c17\"]{text-align:center;min-height:unset !important;--tve-applied-min-height:unset !important;}[data-css=\"tve-u-18636929c19\"]{margin-top:0px !important;margin-bottom:0px !important;}[data-css=\"tve-u-18636929c1a\"]{padding-top:0px !important;padding-bottom:0px 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h1,.tve-leads-conversion-object .thrv_text_element h2,.tve-leads-conversion-object .thrv_text_element h3{margin:0}.tve-leads-conversion-object .thrv_heading h1,.tve-leads-conversion-object .thrv_heading h2,.tve-leads-conversion-object .thrv_heading h3{margin:0;padding:0}.tve-leads-conversion-object .thrv_text_element p,.tve-leads-conversion-object .thrv_text_element h1,.tve-leads-conversion-object .thrv_text_element h2,.tve-leads-conversion-object .thrv_text_element h3{margin:0}<\/style><div class=\"tve-leads-conversion-object\" data-tl-type=\"shortcode_1972\"><div class=\"tve_flt\"><div id=\"tve_editor\" class=\"tve_shortcode_editor\"><div class=\"thrv-leads-form-box tve_no_drag tve_no_icons thrv_wrapper tve_editor_main_content thrv-leads-in-content tve_empty_dropzone\" data-css=\"tve-u-18636929c13\" style=\"\"><div class=\"thrv_wrapper thrv_contentbox_shortcode thrv-content-box\" data-css=\"tve-u-18636929c15\" style=\"--tve-cond-height-m:158; --tve-cond-height-d:68.3594;\"><div class=\"tve-content-box-background\" data-css=\"tve-u-18636929c16\" data-tcb_hover_state_parent=\"1\" style=\"\"><\/div><div class=\"tve-cb\" data-css=\"tve-u-18636929c17\" style=\"\"><div class=\"thrv_wrapper thrv-columns\" data-css=\"tve-u-18636929c19\" style=\"--tcb-col-el-width:475.996;\"><div class=\"tcb-flex-row tcb--cols--1\" data-css=\"tve-u-18636929c1a\"><div class=\"tcb-flex-col\" data-css=\"tve-u-18636929c1b\" style=\"\"><div class=\"tcb-col\" data-css=\"tve-u-18636929c1c\" style=\"\"><div class=\"thrv_wrapper thrv_text_element tve_ea_tl_state_lightbox\" data-css=\"tve-u-18636929c1d\"><p data-css=\"tve-u-18636929c1e\" style=\"text-align: center;\"><a class=\"tve_evt_manager_listen tve_et_click\" data-css=\"tve-u-18636929c1f\" data-tcb-events=\"__TCB_EVENT_[{&quot;a&quot;:&quot;tl_state_lightbox&quot;,&quot;t&quot;:&quot;click&quot;,&quot;config&quot;:{&quot;s&quot;:&quot;14&quot;,&quot;anim&quot;:&quot;instant&quot;}}]_TNEVE_BCT__\" href=\"javascript:void(0)\" target=\"_blank\" style=\"outline: none;\"><span data-css=\"tve-u-18636929c21\"><strong><span style=\"text-decoration: underline;\"><\/span><\/strong><\/span><span data-css=\"tve-u-18636929c22\"><span style=\"text-decoration: underline;\">DOWNLOAD<\/span> FREE hiPSC ILLUSTRATIONS (HD)<\/span><\/a><\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-341770a elementor-widget elementor-widget-spacer\" data-id=\"341770a\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ae204fc elementor-widget elementor-widget-heading\" data-id=\"ae204fc\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\"><p style=\"margin:0cm;text-align:justify\"><b>A protected microenvironment<\/b><span style=\"color: var(--ast-global-color-2);font-size: 2.625rem;font-style: inherit;background-color: var(--ast-global-color-5)\"><\/span><\/p><\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9a88db5 elementor-widget elementor-widget-text-editor\" data-id=\"9a88db5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Before implantation, the fertilized egg and its subsequent totipotent stem cell progeny grow for\u00a0a few days within the zona pellucida &#8211; a 5-10 \u03bcm thick spherical shell composed of glycoproteins. The zona pellucida is a mechanically protective capsule that allows for metabolic exchanges with the environment of the uterus. This structure is hatched around day 7 post-fertilization to enable implantation into the uterine wall. This capsule is then functionally replaced by an extra-embryonic outer layer of trophoblast cells, which provides mechanical protection, enables metabolic exchanges, and creates a controlled 3D niche in which hPSCs can grow exponentially.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-28e0710 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"28e0710\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-dd5cc34\" data-id=\"dd5cc34\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4810186 elementor-widget elementor-widget-spacer\" data-id=\"4810186\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1659b32 elementor-widget elementor-widget-heading\" data-id=\"1659b32\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\"><br><p style=\"margin:0cm;text-align:justify\"><b>A distinctive rosette architecture<\/b><span style=\"color: var(--ast-global-color-2);font-size: 2.625rem;font-style: inherit;background-color: var(--ast-global-color-5)\"><\/span><\/p><\/h3>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d2b300b elementor-widget elementor-widget-text-editor\" data-id=\"d2b300b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>After implantation, hPSCs acquire primed pluripotency and arrange as a spherical single cell-layered epithelium organized around a central cavity. This lumenized rosette architecture is instrumental for the<em> in vivo<\/em> function of the hPSC compartment.\u00a0<\/p><p>First, this radial organization around a central lumen ensures that each hPSC within the colony has similar environmental conditions, including topology, mechanical characteristics and access to oxygen, nutrients, extracellular matrix, and growth factors. Its radial symmetry per se avoids local variations, which could compromise the establishment of ontogenic axes organizing the body plan.\u00a0<\/p><p>Second, in the epiblast rosette, all hPSCs have their apical domains clustered toward the lumen, which acts as central signalling hub, enabling synchronous and homogeneous growth.\u00a0<\/p><p>Finally, the lumenized rosette is a plastic architecture suited for growth. Its roundness suggests a positive intraluminal osmotic pressure. Such a pressure gradient may help expand the surface area of the epithelium as hPSCs divide, and locally decrease the tangential mechanical resistance to accommodate new cells. Facilitating cell division, this conformation also prompts the depletion of abnormal cells, thus safeguarding the genomic integrity of the hPSC colony.<\/p><div>\u00a0<\/div>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b1cfead elementor-widget elementor-widget-shortcode\" data-id=\"b1cfead\" data-element_type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-shortcode\"><div class=\"tve-leads-shortcode tve-leads-triggered tve-tl-anim tl-anim-instant tve-leads-track-shortcode_2015\"><div class=\"tl-style\" id=\"tve_tcb2_2-step-content-upgrade-01_m1\" data-state=\"24\" data-form-state=\"\"><style type=\"text\/css\" class=\"tve_custom_style\">@import url(\"\/\/fonts.googleapis.com\/css?family=Quicksand:300,600,500&subset=latin\");@import url(\"\/\/fonts.googleapis.com\/css?family=Roboto:300,500,700,400,100&subset=latin\");@media 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[data-css=\"tve-u-18636d86bb5\"]{--g-regular-weight:300;--g-bold-weight:600;color:rgb(255,255,255) !important;font-family:Quicksand !important;font-weight:var(--g-bold-weight,bold)!important;text-decoration:none !important;--eff:none!important;--eff-color:rgb(255,255,255)!important;--eff-faded:rgba(255,255,255,0.6)!important;--eff-ensure-contrast:rgba(255,255,255,0.6)!important;--eff-ensure-contrast-complement:rgba(255,255,255,0.6)!important;box-shadow:none !important;border-bottom:none !important;background:none !important;animation:0s ease 0s 1 normal none running none !important;transition:none 0s ease 0s !important;padding-left:0px !important;--tve-applied-box-shadow:none!important;}:not(#tve) [data-css=\"tve-u-18636d86bb5\"] strong{font-weight:600 !important;}[data-css=\"tve-u-18636d86baf\"] > .tcb-flex-col{padding-left:0px;}:not(#tve) [data-css=\"tve-u-18636d86bb5\"]:hover{text-decoration:none !important;background:none !important;box-shadow:none 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.thrv_heading h1,.tve-leads-conversion-object .thrv_heading h2,.tve-leads-conversion-object .thrv_heading h3{margin:0;padding:0}.tve-leads-conversion-object .thrv_text_element p,.tve-leads-conversion-object .thrv_text_element h1,.tve-leads-conversion-object .thrv_text_element h2,.tve-leads-conversion-object .thrv_text_element h3{margin:0}<\/style><div class=\"tve-leads-conversion-object\" data-tl-type=\"shortcode_2015\"><div class=\"tve_flt\"><div id=\"tve_editor\" class=\"tve_shortcode_editor\"><div class=\"thrv-leads-form-box tve_no_drag tve_no_icons thrv_wrapper tve_editor_main_content thrv-leads-in-content tve_empty_dropzone\" data-css=\"tve-u-18636d86ba7\" style=\"\"><div class=\"thrv_wrapper tve_image_caption tve_ea_tl_state_lightbox\" data-css=\"tve-u-18636d86ba8\" style=\"\"><span class=\"tve_image_frame\"><img loading=\"lazy\" decoding=\"async\" class=\"tve_image wp-image-1351 tve_evt_manager_listen tve_et_click\" alt=\"White Paper hiPSC mimicking the lumenized rosette epiblast pluripotent stem cells in vivo\" data-id=\"1351\" width=\"576\" data-init-width=\"2500\" height=\"1600\" data-init-height=\"1600\" title=\"whitebook epiblast\" loading=\"lazy\" src=\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/01\/Horizontal_Book_Mockup_Whitebook.jpg\" data-width=\"576\" data-css=\"tve-u-18636da3ba6\" style=\"\" data-tcb-events=\"__TCB_EVENT_[{&quot;config&quot;:{&quot;anim&quot;:&quot;instant&quot;,&quot;s&quot;:&quot;25&quot;},&quot;a&quot;:&quot;tl_state_lightbox&quot;,&quot;t&quot;:&quot;click&quot;}]_TNEVE_BCT__\"><\/span><\/div><div class=\"thrv_wrapper thrv_contentbox_shortcode thrv-content-box tve_evt_manager_listen tve_et_click tve_ea_tl_state_lightbox\" data-css=\"tve-u-18636d86baa\" style=\"--tve-cond-height-m:158; --tve-cond-height-d:68.3594;\" data-tcb-events=\"__TCB_EVENT_[{&quot;config&quot;:{&quot;anim&quot;:&quot;instant&quot;,&quot;s&quot;:&quot;25&quot;},&quot;a&quot;:&quot;tl_state_lightbox&quot;,&quot;t&quot;:&quot;click&quot;}]_TNEVE_BCT__\"><div class=\"tve-content-box-background\" data-css=\"tve-u-18636d86bac\" data-tcb_hover_state_parent=\"1\" style=\"\"><\/div><div class=\"tve-cb\" data-css=\"tve-u-18636d86bad\" style=\"\"><div class=\"thrv_wrapper thrv-columns\" data-css=\"tve-u-18636d86bae\" style=\"--tcb-col-el-width:581;\"><div class=\"tcb-flex-row tcb--cols--1\" data-css=\"tve-u-18636d86baf\" style=\"\"><div class=\"tcb-flex-col\" data-css=\"tve-u-18636d86bb0\" style=\"\"><div class=\"tcb-col\" data-css=\"tve-u-18636d86bb1\" style=\"\"><div class=\"thrv_wrapper thrv_text_element tve_ea_tl_state_lightbox\" data-css=\"tve-u-18636d86bb2\"><p data-css=\"tve-u-18636d86bb3\" style=\"text-align: center;\"><a class=\"tve_evt_manager_listen tve_et_click\" data-css=\"tve-u-18636d86bb5\" data-tcb-events=\"__TCB_EVENT_[{&quot;a&quot;:&quot;tl_state_lightbox&quot;,&quot;t&quot;:&quot;click&quot;,&quot;config&quot;:{&quot;s&quot;:&quot;25&quot;,&quot;anim&quot;:&quot;instant&quot;}}]_TNEVE_BCT__\" href=\"javascript:void(0)\" target=\"_blank\" style=\"outline: none;\"><span data-css=\"tve-u-18636d86bb6\"><span style=\"text-decoration: underline;\">Download<\/span> White Paper on the hPSC Lumenized Rosette<\/span><\/a><\/p><p><\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Pluripotency is a transient cell property in vivo. At the end of the first week of development, na\u00efve hPSCs transition to primed pluripotency. Within the following 5 to 7 days, the hPSC colony, benefiting from a very fast 15h to 16h cell cycle, grows exponentially to reach over 1,000 cells. <\/p>\n","protected":false},"author":1,"featured_media":1833,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[9],"tags":[],"aioseo_notices":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v21.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>How do human pluripotent stem cells grow in vivo ? - Stem Cells &amp; Cell Therapy Blog I In the Stem Cell Jungle I iPSCs I TreeFrog Therapeutics<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"How do human pluripotent stem cells grow in vivo ? - Stem Cells &amp; Cell Therapy Blog I In the Stem Cell Jungle I iPSCs I TreeFrog Therapeutics\" \/>\n<meta property=\"og:description\" content=\"Pluripotency is a transient cell property in vivo. 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