{"id":2585,"date":"2023-03-07T09:31:01","date_gmt":"2023-03-07T09:31:01","guid":{"rendered":"https:\/\/preprod.treefrog.fr\/stemcelljungle\/?p=2585"},"modified":"2023-03-07T09:33:47","modified_gmt":"2023-03-07T09:33:47","slug":"is-it-possible-to-differentiate-hipscs-into-any-cell-type-in-c-stem","status":"publish","type":"post","link":"https:\/\/preprod.treefrog.fr\/stemcelljungle\/is-it-possible-to-differentiate-hipscs-into-any-cell-type-in-c-stem\/","title":{"rendered":"Is it possible to differentiate hiPSCs into any cell type in C-Stem\u2122?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2585\" class=\"elementor elementor-2585\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9497965 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"9497965\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-53f4d8f\" data-id=\"53f4d8f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f8124ab elementor-widget elementor-widget-text-editor\" data-id=\"f8124ab\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Following a recent peer-reviewed publication describing massive hiPSC expansion in 10L bioreactors with the C-Stem\u2122 technology, Maxime Feyeux, PhD, co-founder and Chief Scientific Officer of TreeFrog Therapeutics, provides insights into iPS differentiation in C-Stem\u2122.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0d52e70 elementor-widget elementor-widget-video\" data-id=\"0d52e70\" data-element_type=\"widget\" data-settings=\"{&quot;youtube_url&quot;:&quot;https:\\\/\\\/youtu.be\\\/yJp2r0pWMjA&quot;,&quot;video_type&quot;:&quot;youtube&quot;,&quot;controls&quot;:&quot;yes&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-wrapper elementor-open-inline\">\n\t\t\t<div class=\"elementor-video\"><\/div>\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-62d0912 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"62d0912\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-40bd1e5\" data-id=\"40bd1e5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d155f1e elementor-widget elementor-widget-text-editor\" data-id=\"d155f1e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Transcript:<\/b><\/p><p>\u00a0<\/p><p>We have another question which is very logical when you think about pluripotent stem cells: Can you differentiate\u00a0the cells in C-Stem\u2122 into any cell type ?<\/p><p>So my answer would be: we do. We&#8217;ve been differentiating liver cells &#8211; so the endoderm &#8211; in the capsule, we&#8217;ve been differentiating hematopoietic stem cells, NK cells from iPSCs in the capsule &#8211; so that would be the mesoderm, and we&#8217;ve also differentiated our main program &#8211; a Parkinson&#8217;s disease cell therapeutic &#8211; which is neural cells in the capsule which would make ectoderm too. So we have the three germ layers, and there is an argument to be made as to why starting from an epiblast-like structure is actually a good idea for differentiation because all the cells have equally access to the medium so they will all react synchronously to the triggers of the medium and differentiation protocols are basically preying on the signals that you put in the medium to differentiate. So we&#8217;ve got very good homogeneity there. And the second thing is that helps scaling it up because then you have this compartmentalized epiblasts everywhere in the bioreactor. You change your medium. Everyone gets equally and at the same time the signals and that helps with having something very reproducible\u00a0and robust to differentiate your cells.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-04e2a5c elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"04e2a5c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-8ae3fd2\" data-id=\"8ae3fd2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-09536d0 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"09536d0\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-md\" href=\"https:\/\/doi.org\/10.1016\/j.biomaterials.2023.122033\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">READ THE PAPER<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Following a recent peer-reviewed publication describing massive hiPSC expansion in 10L bioreactors with the C-Stem\u2122 technology, Maxime Feyeux, PhD, co-founder and Chief Scientific Officer of TreeFrog Therapeutics, provides insights into iPS differentiation in C-Stem\u2122.<\/p>\n","protected":false},"author":1,"featured_media":2587,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"video","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[],"aioseo_notices":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v21.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Is it possible to differentiate hiPSCs into any cell type in C-Stem\u2122? 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