{"id":2148,"date":"2023-02-01T22:08:43","date_gmt":"2023-02-01T22:08:43","guid":{"rendered":"https:\/\/preprod.treefrog.fr\/stemcelljungle\/?p=2148"},"modified":"2023-02-14T11:03:11","modified_gmt":"2023-02-14T11:03:11","slug":"how-do-lumenized-pluripotent-stem-cell-colonies-form-and-what-are-their-properties","status":"publish","type":"post","link":"https:\/\/preprod.treefrog.fr\/stemcelljungle\/how-do-lumenized-pluripotent-stem-cell-colonies-form-and-what-are-their-properties\/","title":{"rendered":"How do lumenized pluripotent stem cell colonies form and what are their properties ?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2148\" class=\"elementor elementor-2148\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-cd65b66 elementor-section-boxed elementor-section-height-default elementor-section-height-default parallax_section_no qode_elementor_container_no\" data-id=\"cd65b66\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6cd26ed\" data-id=\"6cd26ed\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f166942 elementor-widget elementor-widget-text-editor\" data-id=\"f166942\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>The human epiblast is a lumenized and polarized PSC colony<\/strong><\/p><p>The lumenized rosette architecture emerges during the transition from na\u00efve to primed pluripotency. In response to extracellular matrix cues, na\u00efve PSCs start to polarize. As transcriptional factors trigger primed pluripotency, hPSC form<em> de novo<\/em> intracellular actin-rich compartments called apicosomes, which accumulate Ca2+ and express apical proteins. As apicosomes regroup, concomitant mechanisms promoting water influx towards the center of the epiblast participate in the establishment of a fluid-filled lumen. The resulting rosette architecture displays apical-basal polarity: all apical domains are facing the lumen, while basal domains are facing the external environment of the epiblast.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7ae3eca elementor-widget elementor-widget-image\" data-id=\"7ae3eca\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web-1024x576.jpg\" class=\"attachment-large size-large wp-image-1836\" alt=\"Genesis &amp; properties of the pluripotent lumenized rosette\" srcset=\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web-1024x576.jpg 1024w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web-300x169.jpg 300w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web-768x432.jpg 768w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web-1536x864.jpg 1536w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web-700x394.jpg 700w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web-539x303.jpg 539w, https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web.jpg 1920w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3e11470 elementor-widget elementor-widget-text-editor\" data-id=\"3e11470\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Fig. Lumenogenesis &amp; rosette expansion<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6a52c87 elementor-widget elementor-widget-shortcode\" data-id=\"6a52c87\" data-element_type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-shortcode\"><div class=\"tve-leads-shortcode tve-leads-triggered tve-tl-anim tl-anim-instant tve-leads-track-shortcode_1972\"><div class=\"tl-style\" id=\"tve_tcb2_2-step-content-upgrade-01_m1\" data-state=\"12\" data-form-state=\"\"><style type=\"text\/css\" class=\"tve_custom_style\">@import url(\"\/\/fonts.googleapis.com\/css?family=Quicksand:300,600,500&subset=latin\");@import url(\"\/\/fonts.googleapis.com\/css?family=Roboto:300,500,700,400,100&subset=latin\");@media (min-width:300px){[data-css=\"tve-u-18636929c13\"]{overflow:hidden;border-radius:0px !important;background-color:rgba(113,182,213,0) !important;padding:30px 0px !important;margin-top:0px !important;margin-bottom:0px !important;box-shadow:none !important;}[data-css=\"tve-u-18636929c15\"]{--tve-alignment:center;float:none;max-width:516px;margin:0px auto !important;padding:20px !important;}[data-css=\"tve-u-18636929c16\"]{border-radius:50px;overflow:hidden;box-shadow:none;--tve-border-radius:50px;background-color:rgb(21,237,163) !important;--background-color:rgb(21,237,163) !important;--tve-applied-background-color:rgb(21,237,163) !important;}:not(#tve) [data-css=\"tve-u-18636929c15\"]:hover [data-css=\"tve-u-18636929c16\"]{background-color:rgb(0,171,105) !important;--background-color:rgb(0,171,105) !important;--tve-applied-background-color:rgb(0,171,105) !important;}[data-css=\"tve-u-18636929c17\"]{text-align:center;min-height:unset !important;--tve-applied-min-height:unset !important;}[data-css=\"tve-u-18636929c19\"]{margin-top:0px !important;margin-bottom:0px !important;}[data-css=\"tve-u-18636929c1a\"]{padding-top:0px !important;padding-bottom:0px !important;}[data-css=\"tve-u-18636929c1b\"]{max-width:100%;}[data-css=\"tve-u-18636929c1c\"]{text-align:center;}[data-css=\"tve-u-18636929c1d\"]{overflow:hidden;max-width:608px;padding:0px !important;border-radius:5px !important;}:not(#tve) [data-css=\"tve-u-18636929c1e\"]{color:rgb(255,255,255) !important;font-size:20px !important;font-family:Roboto !important;font-weight:300 !important;padding-bottom:0px !important;margin-bottom:0px !important;}[data-css=\"tve-u-18636929c1e\"] strong{font-weight:500 !important;}:not(#tve) [data-css=\"tve-u-18636929c1f\"]{--g-regular-weight:300;--g-bold-weight:600;color:rgb(255,255,255) !important;font-family:Quicksand !important;font-weight:var(--g-bold-weight,bold) !important;text-decoration-line:none !important;--eff:none !important;text-decoration-color:var(--eff-color,currentColor) !important;}:not(#tve) [data-css=\"tve-u-18636929c1f\"] strong{font-weight:600 !important;}}@media (max-width:1023px){[data-css=\"tve-u-18636929c13\"]{padding-left:10px !important;padding-right:10px !important;}[data-css=\"tve-u-18636929c17\"]{min-height:unset !important;--tve-applied-min-height:unset !important;}[data-css=\"tve-u-18636929c1d\"]{float:none;width:100%;margin-left:auto !important;margin-right:auto !important;}:not(#tve) [data-css=\"tve-u-18636929c1f\"]{font-size:22px !important;}}@media (max-width:767px){[data-css=\"tve-u-18636929c17\"]{min-height:unset !important;--tve-applied-min-height:unset !important;}[data-css=\"tve-u-18636929c1d\"]{margin-top:0px !important;}:not(#tve) [data-css=\"tve-u-18636929c1f\"]{font-size:20px !important;}}<\/style><style type=\"text\/css\" class=\"tve_user_custom_style\">.tve-leads-conversion-object .thrv_heading h1,.tve-leads-conversion-object .thrv_heading h2,.tve-leads-conversion-object .thrv_heading h3{margin:0;padding:0}.tve-leads-conversion-object .thrv_text_element p,.tve-leads-conversion-object .thrv_text_element h1,.tve-leads-conversion-object .thrv_text_element h2,.tve-leads-conversion-object .thrv_text_element h3{margin:0}.tve-leads-conversion-object .thrv_heading h1,.tve-leads-conversion-object .thrv_heading h2,.tve-leads-conversion-object .thrv_heading h3{margin:0;padding:0}.tve-leads-conversion-object .thrv_text_element p,.tve-leads-conversion-object .thrv_text_element h1,.tve-leads-conversion-object .thrv_text_element h2,.tve-leads-conversion-object .thrv_text_element h3{margin:0}<\/style><div class=\"tve-leads-conversion-object\" data-tl-type=\"shortcode_1972\"><div class=\"tve_flt\"><div id=\"tve_editor\" class=\"tve_shortcode_editor\"><div class=\"thrv-leads-form-box tve_no_drag tve_no_icons thrv_wrapper tve_editor_main_content thrv-leads-in-content tve_empty_dropzone\" data-css=\"tve-u-18636929c13\" style=\"\"><div class=\"thrv_wrapper thrv_contentbox_shortcode thrv-content-box\" data-css=\"tve-u-18636929c15\" style=\"--tve-cond-height-m:158; --tve-cond-height-d:68.3594;\"><div class=\"tve-content-box-background\" data-css=\"tve-u-18636929c16\" data-tcb_hover_state_parent=\"1\" style=\"\"><\/div><div class=\"tve-cb\" data-css=\"tve-u-18636929c17\" style=\"\"><div class=\"thrv_wrapper thrv-columns\" data-css=\"tve-u-18636929c19\" style=\"--tcb-col-el-width:475.996;\"><div class=\"tcb-flex-row tcb--cols--1\" data-css=\"tve-u-18636929c1a\"><div class=\"tcb-flex-col\" data-css=\"tve-u-18636929c1b\" style=\"\"><div class=\"tcb-col\" data-css=\"tve-u-18636929c1c\" style=\"\"><div class=\"thrv_wrapper thrv_text_element tve_ea_tl_state_lightbox\" data-css=\"tve-u-18636929c1d\"><p data-css=\"tve-u-18636929c1e\" style=\"text-align: center;\"><a class=\"tve_evt_manager_listen tve_et_click\" data-css=\"tve-u-18636929c1f\" data-tcb-events=\"__TCB_EVENT_[{&quot;a&quot;:&quot;tl_state_lightbox&quot;,&quot;t&quot;:&quot;click&quot;,&quot;config&quot;:{&quot;s&quot;:&quot;14&quot;,&quot;anim&quot;:&quot;instant&quot;}}]_TNEVE_BCT__\" href=\"javascript:void(0)\" target=\"_blank\" style=\"outline: none;\"><span data-css=\"tve-u-18636929c21\"><strong><span style=\"text-decoration: underline;\"><\/span><\/strong><\/span><span data-css=\"tve-u-18636929c22\"><span style=\"text-decoration: underline;\">DOWNLOAD<\/span> FREE hiPSC ILLUSTRATIONS (HD)<\/span><\/a><\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ac20c13 elementor-widget elementor-widget-text-editor\" data-id=\"ac20c13\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Lumen formation is required for optimal proliferation and homogenous pluripotency<\/b><\/p><p><b>\u00a0<\/b><\/p><p>hPSCs in lumenized rosette conformation are characterized by prolonged undifferentiated proliferation without undergoing senescence or quiescence. Kim <i>et al.<\/i> showed in 20211 that inhibiting lumen formation in PSC colonies results in reduced proliferation, and that the lumen is required for the establishment of critical signalling pathways, such as the Nodal cascade. Although the composition of the luminal fluid and the signalling molecules found within this central cavity are not fully characterized, the lumen is known to enhance intercellular communication within the rosette, and this architecture is considered to contribute to the maintenance of stemness during hPSC exponential growth. In a 2021 interview, developmental biologist Marta Shahbazi reported that in vitro, PSCs that escape the 3D rosette architecture are differentiating, thus losing their pluripotent phenotype.<\/p><p>\u00a0<\/p><p><b>The rosette architecture participates in the fitness of pluripotent stem cells<\/b><\/p><p>\u00a0<\/p><p>In 2018, Knouse <em>et al.<\/em> demonstrated that polarized epithelial tissue architecture is required for high-fidelity chromosome segregation. Loss of polarity and disruption of tissue architecture around a central lumen are in fact correlated with mitotic failures, aneuploidy and accumulation of DNA damage in epithelial cells. This observation gains a particular relevance in the context of early human development, where genomic abnormalities are extremely common. In 2020, a single-cell RNA sequencing analysis reported that more than 70% of human embryos contain karyotypically abnormal cells at the pre-implantation stage, prior to epiblast formation.<\/p><p>Recent research has shown that the pluripotent rosette undergoes natural quality control through cell competition mechanisms, leading to the elimination of abnormal cells through apoptosis and autophagy In the mouse epiblast, 35% of PSCs were found to be eliminated by the onset of gastrulation.\u00a0 Several groups demonstrated that defective cells, harboring mis-patterning, karyotypic, or mitochondrial abnormalities are efficiently depleted in epiblast conformation. Normal diploid PSCs were also found to increase their proliferation to compensate for the elimination of abnormal cells in the epiblast. Overall, the competition between PSCs in the epiblast, which involves YAP\/TAZ25, MYC23, p5324, and mTOR signalling pathways, is considered as a critical mechanism, safeguarding pluripotency and genomic integrity before germline commitment.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d10f506 elementor-widget elementor-widget-shortcode\" data-id=\"d10f506\" data-element_type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-shortcode\"><div class=\"tve-leads-shortcode tve-leads-triggered tve-tl-anim tl-anim-instant tve-leads-track-shortcode_2015\"><div class=\"tl-style\" id=\"tve_tcb2_2-step-content-upgrade-01_m1\" data-state=\"24\" data-form-state=\"\"><style type=\"text\/css\" class=\"tve_custom_style\">@import url(\"\/\/fonts.googleapis.com\/css?family=Quicksand:300,600,500&subset=latin\");@import url(\"\/\/fonts.googleapis.com\/css?family=Roboto:300,500,700,400,100&subset=latin\");@media (min-width:300px){[data-css=\"tve-u-18636d86ba7\"]{overflow:hidden;border-radius:0px !important;background-color:rgba(113,182,213,0) !important;padding:30px 0px !important;margin-top:0px !important;margin-bottom:0px !important;box-shadow:none !important;}[data-css=\"tve-u-18636d86ba8\"]{width:72%;float:none;z-index:3;position:relative;--tve-alignment:center;margin-left:auto !important;margin-right:auto !important;}[data-css=\"tve-u-18636d86baa\"]{--tve-alignment:center;float:none;max-width:621px;margin:0px auto !important;padding:20px !important;}[data-css=\"tve-u-18636d86bac\"]{border-radius:50px;overflow:hidden;box-shadow:none;--tve-border-radius:50px;background-color:rgb(21,237,163) !important;--background-color:rgb(21,237,163)!important;--tve-applied-background-color:rgb(21,237,163)!important;}:not(#tve) [data-css=\"tve-u-18636d86baa\"]:hover [data-css=\"tve-u-18636d86bac\"]{background-color:rgb(0,171,105) !important;--background-color:rgb(0,171,105)!important;--tve-applied-background-color:rgb(0,171,105)!important;}[data-css=\"tve-u-18636d86bad\"]{text-align:center;min-height:unset !important;--tve-applied-min-height:unset !important;}[data-css=\"tve-u-18636d86bae\"]{margin-top:0px !important;margin-bottom:0px !important;}[data-css=\"tve-u-18636d86baf\"]{padding-top:0px !important;padding-bottom:0px !important;margin-left:0px !important;}[data-css=\"tve-u-18636d86bb0\"]{max-width:100%;}[data-css=\"tve-u-18636d86bb1\"]{text-align:center;}[data-css=\"tve-u-18636d86bb2\"]{overflow:hidden;max-width:608px;padding:0px !important;border-radius:5px !important;}:not(#tve) [data-css=\"tve-u-18636d86bb3\"]{color:rgb(255,255,255) !important;font-size:20px !important;font-family:Roboto !important;font-weight:300 !important;padding-bottom:0px !important;margin-bottom:0px !important;}[data-css=\"tve-u-18636d86bb3\"] 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.thrv_heading h1,.tve-leads-conversion-object .thrv_heading h2,.tve-leads-conversion-object .thrv_heading h3{margin:0;padding:0}.tve-leads-conversion-object .thrv_text_element p,.tve-leads-conversion-object .thrv_text_element h1,.tve-leads-conversion-object .thrv_text_element h2,.tve-leads-conversion-object .thrv_text_element h3{margin:0}<\/style><div class=\"tve-leads-conversion-object\" data-tl-type=\"shortcode_2015\"><div class=\"tve_flt\"><div id=\"tve_editor\" class=\"tve_shortcode_editor\"><div class=\"thrv-leads-form-box tve_no_drag tve_no_icons thrv_wrapper tve_editor_main_content thrv-leads-in-content tve_empty_dropzone\" data-css=\"tve-u-18636d86ba7\" style=\"\"><div class=\"thrv_wrapper tve_image_caption tve_ea_tl_state_lightbox\" data-css=\"tve-u-18636d86ba8\" style=\"\"><span class=\"tve_image_frame\"><img loading=\"lazy\" decoding=\"async\" class=\"tve_image wp-image-1351 tve_evt_manager_listen tve_et_click\" alt=\"White Paper hiPSC mimicking the lumenized rosette epiblast pluripotent stem cells in vivo\" data-id=\"1351\" width=\"576\" data-init-width=\"2500\" height=\"1600\" data-init-height=\"1600\" title=\"whitebook epiblast\" loading=\"lazy\" src=\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/01\/Horizontal_Book_Mockup_Whitebook.jpg\" data-width=\"576\" data-css=\"tve-u-18636da3ba6\" style=\"\" data-tcb-events=\"__TCB_EVENT_[{&quot;config&quot;:{&quot;anim&quot;:&quot;instant&quot;,&quot;s&quot;:&quot;25&quot;},&quot;a&quot;:&quot;tl_state_lightbox&quot;,&quot;t&quot;:&quot;click&quot;}]_TNEVE_BCT__\"><\/span><\/div><div class=\"thrv_wrapper thrv_contentbox_shortcode thrv-content-box tve_evt_manager_listen tve_et_click tve_ea_tl_state_lightbox\" data-css=\"tve-u-18636d86baa\" style=\"--tve-cond-height-m:158; --tve-cond-height-d:68.3594;\" data-tcb-events=\"__TCB_EVENT_[{&quot;config&quot;:{&quot;anim&quot;:&quot;instant&quot;,&quot;s&quot;:&quot;25&quot;},&quot;a&quot;:&quot;tl_state_lightbox&quot;,&quot;t&quot;:&quot;click&quot;}]_TNEVE_BCT__\"><div class=\"tve-content-box-background\" data-css=\"tve-u-18636d86bac\" data-tcb_hover_state_parent=\"1\" style=\"\"><\/div><div class=\"tve-cb\" data-css=\"tve-u-18636d86bad\" style=\"\"><div class=\"thrv_wrapper thrv-columns\" data-css=\"tve-u-18636d86bae\" style=\"--tcb-col-el-width:581;\"><div class=\"tcb-flex-row tcb--cols--1\" data-css=\"tve-u-18636d86baf\" style=\"\"><div class=\"tcb-flex-col\" data-css=\"tve-u-18636d86bb0\" style=\"\"><div class=\"tcb-col\" data-css=\"tve-u-18636d86bb1\" style=\"\"><div class=\"thrv_wrapper thrv_text_element tve_ea_tl_state_lightbox\" data-css=\"tve-u-18636d86bb2\"><p data-css=\"tve-u-18636d86bb3\" style=\"text-align: center;\"><a class=\"tve_evt_manager_listen tve_et_click\" data-css=\"tve-u-18636d86bb5\" data-tcb-events=\"__TCB_EVENT_[{&quot;a&quot;:&quot;tl_state_lightbox&quot;,&quot;t&quot;:&quot;click&quot;,&quot;config&quot;:{&quot;s&quot;:&quot;25&quot;,&quot;anim&quot;:&quot;instant&quot;}}]_TNEVE_BCT__\" href=\"javascript:void(0)\" target=\"_blank\" style=\"outline: none;\"><span data-css=\"tve-u-18636d86bb6\"><span style=\"text-decoration: underline;\">Download<\/span> White Paper on the hPSC Lumenized Rosette<\/span><\/a><\/p><p><\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>The lumenized rosette architecture emerges during the transition from na\u00efve to primed pluripotency. In response to extracellular matrix cues, na\u00efve PSCs start to polarize. As transcriptional factors trigger primed pluripotency, hPSC form de novo intracellular actin-rich compartments called apicosomes, which accumulate Ca2+ and express apical proteins. As apicosomes regroup, concomitant mechanisms promoting water influx towards the center of the epiblast participate in the establishment of a fluid-filled lumen. The resulting rosette architecture displays apical-basal polarity: all apical domains are facing the lumen, while basal domains are facing the external environment of the epiblast.<\/p>\n","protected":false},"author":1,"featured_media":1836,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[9],"tags":[],"aioseo_notices":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v21.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>How do lumenized pluripotent stem cell colonies form and what are their properties ? - Stem Cells &amp; Cell Therapy Blog I In the Stem Cell Jungle I iPSCs I TreeFrog Therapeutics<\/title>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"How do lumenized pluripotent stem cell colonies form and what are their properties ? - Stem Cells &amp; Cell Therapy Blog I In the Stem Cell Jungle I iPSCs I TreeFrog Therapeutics\" \/>\n<meta property=\"og:description\" content=\"The lumenized rosette architecture emerges during the transition from na\u00efve to primed pluripotency. In response to extracellular matrix cues, na\u00efve PSCs start to polarize. As transcriptional factors trigger primed pluripotency, hPSC form de novo intracellular actin-rich compartments called apicosomes, which accumulate Ca2+ and express apical proteins. As apicosomes regroup, concomitant mechanisms promoting water influx towards the center of the epiblast participate in the establishment of a fluid-filled lumen. The resulting rosette architecture displays apical-basal polarity: all apical domains are facing the lumen, while basal domains are facing the external environment of the epiblast.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/how-do-lumenized-pluripotent-stem-cell-colonies-form-and-what-are-their-properties\/\" \/>\n<meta property=\"og:site_name\" content=\"Stem Cells &amp; Cell Therapy Blog I In the Stem Cell Jungle I iPSCs I TreeFrog Therapeutics\" \/>\n<meta property=\"article:published_time\" content=\"2023-02-01T22:08:43+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-02-14T11:03:11+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/wp-content\/uploads\/sites\/5\/2023\/02\/lumenogenesis-pluripotent-stem-cell-epiblast-rosette-TreeFrog-In-The-Stem-Cell-Jungle-web.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1920\" \/>\n\t<meta property=\"og:image:height\" content=\"1080\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Piergueritona\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@treefrogtx\" \/>\n<meta name=\"twitter:site\" content=\"@treefrogtx\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/how-do-lumenized-pluripotent-stem-cell-colonies-form-and-what-are-their-properties\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/how-do-lumenized-pluripotent-stem-cell-colonies-form-and-what-are-their-properties\/\"},\"author\":{\"name\":\"Piergueritona\",\"@id\":\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/#\/schema\/person\/ab868aec652cf07c221b48c1dc91a220\"},\"headline\":\"How do lumenized pluripotent stem cell colonies form and what are their properties ?\",\"datePublished\":\"2023-02-01T22:08:43+00:00\",\"dateModified\":\"2023-02-14T11:03:11+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/how-do-lumenized-pluripotent-stem-cell-colonies-form-and-what-are-their-properties\/\"},\"wordCount\":478,\"publisher\":{\"@id\":\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/#organization\"},\"articleSection\":[\"Articles\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/how-do-lumenized-pluripotent-stem-cell-colonies-form-and-what-are-their-properties\/\",\"url\":\"https:\/\/preprod.treefrog.fr\/stemcelljungle\/how-do-lumenized-pluripotent-stem-cell-colonies-form-and-what-are-their-properties\/\",\"name\":\"How do lumenized pluripotent stem cell colonies form and what are their properties ? 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